关键词: font-size:medium, ">黄曲霉毒素；胖大海；标准加入法；标准曲线法；高效液相-柱后光化学衍生法；超高效液相色谱-串联质谱法；局限性

Abstract: Objective： The content of aflatoxin B1 in Sterculiae Lychnophorae Semen was determined by the standard addition method， and the advantages and limitations of this method were analyzed. Methods： Using high-performance liquid chromatography with post-column photochemical derivatization (HPLC-FLD) as the detection method， the standard addition method was analyzed the content of aflatoxin B1 in Sterculiae Lychnophorae Semen. To verify the accuracy and reliability of this method， the same batch of samples were simultaneously determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)， and the results of the two methods were compared and analyzed. Results： The standard addition method can reduce the loss and interference caused by the sample pretreatment process and effectively offset the matrix effect. The method is accurate and reliable. However， the method also has some shortcomings， such as large sample consumption， high requirements for experimental operation and linear range， making it less suitable for batch analysis， and scenarios with limited sample availability. Conclusion： The standard addition method can effectively overcome the influence of matrix effect. This method can be used as a supplement to the external standard method to effectively improve the accuracy of the determination of aflatoxin B1 content in Sterculiae Lychnophorae Semen， though it still presents certain limitations in terms of operational complexity and applicability.

Key words: font-size:medium, ">Aflatoxins； Sterculiae Lychnophorae Semen； Standard addition method； Standard curve method； HPLC-FLD； UPLC-MS/MS； Limitations